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. 2015 Apr 28;12:35. doi: 10.1186/s12977-015-0162-8

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© Macfarlane and Badge; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Detection of structural variation at HERV-K(HML-2) loci. (A) Formation of alleles and strategy for independent amplification of structural variations in novel human HERV-K(HML-2) proviral loci. Primer binding sites are indicated by arrows (). The locus specific primer combination a + b amplifies the pre-insertion site and solo LTR. Primer combination a + GAG amplifies the 5′ LTR of a full-length provirus and ENV + b the 3′ LTR. (B) Composite agarose gels of locus specific amplification for allelic variation. Numbers on the left correspond to the co-migrating DNA size marker (kb). Samples are the same as used in Figure 1D, with humans in a different order. The identity of each band was confirmed by DNA sequencing. Refer to Additional file 7 for un-cropped gel images.

Inline graphic — Detection of structural variation at HERV-K(HML-2) loci. (A) Formation of alleles and strategy for independent amplification of structural variations in novel human HERV-K(HML-2) proviral loci. Primer binding sites are indicated by arrows (). The locus specific primer combination a + b amplifies the pre-insertion site and solo LTR. Primer combination a + GAG amplifies the 5′ LTR of a full-length provirus and ENV + b the 3′ LTR. (B) Composite agarose gels of locus specific amplification for allelic variation. Numbers on the left correspond to the co-migrating DNA size marker (kb). Samples are the same as used in Figure 1D, with humans in a different order. The identity of each band was confirmed by DNA sequencing. Refer to Additional file 7 for un-cropped gel images.