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. 2014 Aug 5;14:211. doi: 10.1186/s12870-014-0211-9

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Copyright © 2014 Hao et al.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Transient expression of thefla_LsoinN. benthamiana.N. benthamiana leaves were inoculated with A. tumefaciens GV3101 containing pBin:fla_Lso. The infiltrated tissues were harvested at 1, 2, 3, 4 and 8 days post-infiltration (dpi) for RNA isolation and cDNA preparation. RT-PCR (A) and RT-qPCR (B) were performed to determine gene expression of the fla_Lso at different time after infiltration. For RT-qPCR, the samples were normalized against NbEF1α. Data were shown as average fold change from three independent experiments.