Table 2.
Serum concentrations of cytokines from untreated, DMBA Only- and DMBA + ROSI-treated strains
| PPARγ-WT | PPARγ-MG KO | |||||
|---|---|---|---|---|---|---|
| Untreated (n = 5) | DMBA Only (n = 4) | DMBA + ROSI (n = 4) | Untreated (n = 5) | DMBA Only (n = 4) | DMBA + ROSI (n = 4) | |
| Cytokine [signif] | mean ± SD; all values expressed as pg / ml | |||||
| IL-1α | 90.6 ± 56.6 | 93.9 ± 106.3 | 48.9 ± 32.9 | 177.0 ± 206.4 | 39.8 ± 66.1 | 36.8 ± 46.0 |
| IL-1β | 372.6 ± 105.3 | 3016.0 ± 5279.0 | 309.0 ± 74.0 | 195.4 ± 25.9 | 870.9 ± 1383.0 | 116.7 ± 91.1 |
| IL-2 | 62.8 ± 47.0 | 42.4 ± 27.9 | 64.6 ± 80.3 | 34.9 ± 15.0 | 24.8 ± 6.1 | 41.8 ± 32.9 |
| IL-3 | 6.8 ± 15.2 | 63.4 ± 111.5 | 16.0 ± 13.6 | 12.9 ± 6.6 | 25.4 ± 40.9 | 6.8 ± 5.6 |
| IL-4 [gg] | 16.1 ± 7.9 | 16.4 ± 3.2 | 20.1 ± 10.0 | 11.8 ± 1.5 | 10.4 ± 1.2 | 11.0 ± 1.1 |
| IL-5 | 40.0 ± 16.3 | 280.7 ± 454.0 | 23.2 ± 17.2 | ND | 77.4 ± 154.8 | 4.6 ± 9.3 |
| IL-6 | 18.0 ± 16.4 | 215.5 ± 387.5 | 20.7 ± 12.0 | 5.7 ± 1.5 | 62.7 ± 106.6 | 14.5 ± 8.5 |
| IL-9 | ND | ND | ND | ND | ND | ND |
| IL-10 [ggg] | 97.3 ± 64.6 | 163.2 ± 114.2 | 117.6 ± 48.8 | 23.5 ± 9.5 | 39.7 ± 19.9 | 52.0 ± 20.9 |
| IL-12(p40) [tt] | 694.9 ± 299.5 | 1212.0 ± 302.4 | 625.3 ± 314.0 | 554.5 ± 176.1 | 971.7 ± 306.8 | 665.1 ± 227.3 |
| IL-12(p70) | ND | ND | ND | ND | ND | ND |
| IL-13 [g] | 654.1 ± 638.6 | 410.6 ± 437.3 | 440.6 ± 380.4 | 95.4 ± 71.7 | 124.9 ± 33.2 | 248.0 ± 91.7 |
| IL-17A | 32.3 ± 23.8 | 588.5 ± 1114.0 | 34.9 ± 19.5 | 2.1 ± 3.2 | 150.3 ± 294.5 | 7.8 ± 12.6 |
| Eotaxin [gg] | 1552.0 ± 1593.0 | 1141.0 ± 890.4 | 2016.0 ± 743.7 | 400.4 ± 397.5 | 620.8 ± 482.6 | 188.4 ± 163.4 |
| G-CSF | 199.6 ± 85.9 | 1631.0 ± 929.1 | 3393.0 ± 5274.0 | 149.1 ± 46.6 | 564.3 ± 375.5 | 433.7 ± 121.9 |
| GM-CSF [gggg] | 420.2 ± 218.5 | 384.4 ± 86.8 | 375.3 ± 94.4 | 173.0 ± 68.1 * | 148.6 ± 47.4 | 110.1 ± 75.6 Δ |
| IFN-γ [g] | 35.4 ± 38.6 | 26.6 ± 10.5 | 23.9 ± 11.3 | 15.3 ± 6.8 | 6.8 ± 6.4 | 9.6 ± 7.0 |
| KC | 46.4 ± 62.2 | 25.3 ± 9.4 | 41.1 ± 28.2 | 8.8 ± 1.6 | 23.1 ± 11.5 | 14.9 ± 2.4 |
| MCP-1 | 218.2 ± 132.6 | 1062.0 ± 1493.0 | 286.4 ± 156.2 | 75.8 ± 38.7 | 190.7 ± 132.6 | 95.9 ± 17.7 |
| MIP-1α [gg] | 67.2 ± 47.2 | 87.8 ± 38.9 | 82.5 ± 54.7 | 45.1 ± 15.2 | 26.3 ± 8.9 | 37.1 ± 11.4 |
| MIP-1β | 51.9 ± 36.9 | 525.1 ± 909.0 | 39.4 ± 13.2 | 15.2 ± 4.0 | 185.8 ± 335.4 | 17.4 ± 15.8 |
| RANTES | 31.5 ± 23.1 | 71.6 ± 76.2 | 40.7 ± 19.4 | 37.1 ± 8.5 | 82.1 ± 97.6 | 27.2 ± 3.9 |
| TNF-α | 1128.0 ± 992.2 | 19244.0 ± 36869.0 | 538.0 ± 207.5 | 272.8 ± 81.9 | 5763.0 ± 10948.0 | 363.3 ± 352.8 |
| VEGF | 144.6 ± 32.8 (7) | 489.7 ± 420.8 (3) ** | 132.8 ± 33.7 (4) # | 254.4 ± 57.0 (8) | 167.5 ± 40.9 (4) # | 137.3 ± 22.4 (4) |
| Leptin | 22290.0 ± 11260.0 (7) | 8040.0 ± 1968.0 (3) | 25820.0 ± 18020.0 (4) | 13680.0 ± 1899.9 (8) | 32140.0 ± 40310.0 (4) | 39560.0 ± 44040.0 (4) |
| PGE Metabolites | 560.1 ± 676.7 (5) | 76.7 ± 13.7 (4) | 93.9 ± 51.6 (4) | 141.3 ± 122.2 (4) | 335.0 ± 158.5 (3) | 229.0 ± 202.7 (3) |
Concentrations reported as mean ± standard deviation (SD) and expressed as pg/ml. Except for VEGF, leptin, and PGE metabolites, which were analyzed with separate ELISA kits, all cytokine concentrations were obtained by a multiplex array. *, significantly different from Untreated PPARγ-WT, p < 0.05; **, significantly different from Untreated PPARγ-WT, p < 0.01; #, significantly different from DMBA Only-treated PPARγ-WT, p < 0.05; Δ, significantly different from DMBA + ROSI-treated PPARγ-WT, p < 0.05. g, genotype different, p < 0.05; gg, genotype different, p < 0.01; ggg, genotype different, p < 0.001; gggg, genotype different, p < 0.0001; tt, treatment different p < 0.01; ND, not detectable.