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. 2014 Jul 18;13:113. doi: 10.1186/s12933-014-0113-z

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Copyright © 2014 Yu et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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p38mapk is involved in Arg-II-induced eNOS-uncoupling. (A) In cultured HUVECs, overexpressing Arg-II gene led to elevated p38mapk activation as measured by the Thr180/Tyr182 phosphorylated p38mapk (p-p38mapk). n = 6, **p < 0.01 vs Con group. (B) HUVECs were transduced first with rAd/U6-LacZ^shRNA as control or rAd/U6-p38^shRNA. Twelve hours after the 1^st transduction with the rAd/U6-shRNAs, the cells were then transduced either with rAd/CMV as control (Con) or with rAd/CMV-Arg-II to overexpress Arg-II (Arg-II). Experiments were performed 60 hours post the 2^nd transduction (48 hours in 5% FCS-RPMI-1640 medium plus overnight serum-starvation in 0.2% FCS-RPMI-1640). Shown are representative images of four independent experiments. ***p < 0.005 vs Con/LacZ group, ^†††p < 0.005 vs Arg-II/LacZ group. Scale bar = 0.2 mm.