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A, Fluorescently labeled circulating tumor cells (red, cytokeratin; green, CD45; blue, 4′, 6-diamidino-2-phenylindole; original magnification ×60, digitally enlarged). B, Same circulating tumor cell, re-stained with Wright-Giemsa (original magnification ×100 oil). C, A similar cell from the original biopsy 4 years prior with arrow pointing to lobated nucleus (Diff-Quick, original magnification ×100 oil).
