Figure 8.

LPS induces Src-mediated tyrosine phosphorylation of p18. LMVECs were exposed to LPS (1 μg/ml, 6 h) in the presence or absence of PP2 pretreatment (12 µM, 30 min). A) LMVECs were lysed and subjected to immunoblot analysis for phosphorylated Src. Blots were stripped and reprobed for total Src levels. A representative blot (inset) and densitometry analysis are shown. B) Early endosomes were isolated from LMVECs via immunoprecipitation with an antibody specific to the EEA1 and subjected to immunoblot analysis with an antibody specific to Src. Blots were stripped and reprobed for EEA1. Representative blots are shown. C) Lysates were immunoprecipitated for tyrosine phosphorylation using the 4G10 clone antibody. Immunocomplexes and whole-cell lysates were subjected to immunoblot analysis with an antibody specific to p18 and actin, respectively. A representative blot (inset) and densitometry analysis are shown. Data are presented as the mean ± SD (n = 4). *P < 0.05 vs. vehicle.