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. 2014 Nov 17;29(3):894–910. doi: 10.1096/fj.14-255729

Figure 4.

Figure 4.

LCARKO Leydig cells reach final adult number but do not express mature Leydig cell markers. A) The total number of Leydig cells per testis is not significantly different between controls and LCARKOs at d 21, 35, and 80. Error bars, sem. B) The percentage of testis occupied by Leydig cells (staining for HSD3B) appears to be larger in the LCARKO than in the control. Scale bars, 100 µm. C) The size of Leydig cell cytoplasm appears larger in LCARKOs than controls at d 80 (lines delineate seminiferous tubules in control panel; arrows indicate interstitium). Scale bars, 20 µm. D) Hsd3b6 is significantly decreased (****P < 0.0001) in LCARKO testis compared to controls at d 80 and is absent in ARKO testis. E) Ptgds is significantly decreased (****P < 0.0001) in LCARKO testis compared to controls at d 80 and is absent in ARKO testis. F) Insl3 is significantly decreased (****P < 0.0001) in LCARKO testis compared to controls at d 80 and is absent in ARKO testis. G) INSL3 staining is widespread throughout the interstitium of control testes, but LCARKO testes show 2 populations of cells with Leydig cell-like morphology: a larger population that does not stain for INSL3 (arrowhead); and a smaller population that does (arrows). Scale bars, 20 µm.