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. 2014 Nov 17;29(3):894–910. doi: 10.1096/fj.14-255729

Figure 5.

Figure 5.

LCARKO testes have changes in steroidogenic enzyme expression but produce normal levels of T. A) Cyp17a1 gene expression is significantly decreased (****P < 0.0001) in LCARKO testis compared to controls at d 80 and is absent in ARKO testis. B) AR immunostaining (green) is seen in the nuclei of CYP17A1 (blue)-positive Leydig cells in controls (arrowhead). Both AR-positive (arrowhead) and -negative (arrow) cells in LCARKOs express CYP17A1. Scale bars, 20 µm. C) Hsd17b3 gene expression is significantly decreased (**P < 0.01) in LCARKO testis compared to controls at d 80 and is absent in ARKO testis. D) Two populations of Leydig cells can be seen in the LCARKO: an HSD17B3-positive population (arrowheads), and an HSD17B3-negative population (arrow). Scale bars, 20 μm E) AR immunostaining (red) is seen in the nuclei of HSD17B3 (blue)-positive Leydig cells in controls. In LCARKOs, HSD17B3 is localized to AR-positive cells (arrowhead), but not AR-negative cells (arrow). Scale bars, 20 µm. F) Hsbd3b1 gene expression is significantly increased (**P < 0.01) in LCARKO testis compared to controls at d 80. G) Cyp11a1 gene expression is significantly increased (***P < 0.001) in LCARKO testis compared to controls at d 80. H) Intratesticular progesterone is significantly increased (**P < 0.01) in LCARKO compared to control mice at d 80. I) Plasma T levels are not significantly different between control and LCARKO mice at d 80, d 100 hCG treated, and 7–12 mo hCG treated. J) Plasma LH levels are not significantly different between control and LCARKO mice at d 80. K) ITT levels are not significantly different between control and LCARKO testes at d 80.