Figure 5.

STAT3C-transduced T cells express an effector phenotype. Primary human CD4⁺ T cells were retrovirally transduced either with human STAT3C (red), FOXP3 (blue), or an empty control vector (black). Transduced (GFP⁺) T cells were analyzed for (A) intracellular expression of granzyme B. Gating strategies are shown in the upper panels. Nontransduced (left) and transduced T cells (right) are depicted. B) Intracellular expression of granzyme B. Left panel shows box plots of mean fluorescence intensity; right panel shows box plots of percent positive cells of CD4⁺ T cells transduced with the indicated transgenes (resting phase) and stained for intracellular granzyme B expression. Data are representative of 5 healthy donors. *P < 0.05; **P < 0.01. C) Staining for surface expression of the regulatory T cell markers CD25, CD39, CD127, LAG3, and CD49b and intracellular expression of CD152/CTLA-4. One representative experiment of 5 is shown. Thin gray lines represent staining with isotype-matched control mAb.