Figure 5.

Trafficking of transducin in Rp2h^−/− photoreceptors and of ODR-3 in mutant C. elegans. A–C) Normal trafficking to rod outer segments of Tα in dark-adapted retina (A), translocation to the inner segments in light (B), and return of Tα to photoreceptor outer segments during dark adaptation (C). Rp2h^−/− mice were illuminated with light of 2000 lux for 40 min and returned to the dark as described previously (27). After 10 h in the dark, mouse eyeballs were harvested. Frozen sections were stained with anti-Tα antibody. D, E) WT and Rp2h^−/− retina frozen sections (age of 1 mo) were stained with anti-Tβ antibody. F, G) Defect in transport of ODR-3 to olfactory cilia in rpi-2 mutant C. elegans. The WT and rpi-2 mutant C. elegans were stained with anti-ODR-3. Arrows point at mislocalized ODR-3 in the cell membrane.