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. 2015 May 6;16(1):357. doi: 10.1186/s12864-015-1594-1

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© Hermsen et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Substrain variant characteristics. (a) Bar plots showing the contribution of each nucleotide change for all substrain variants (observed) versus the control variants (expected). Error bars represent the 95% confidence interval. (b) Bar plot showing the K_a/K_s ratio ratio of the substrain variants versus the control variants. (c) Bar plot showing the average phastCons score for each substrain variant compared to the control variants. Substrain variants affect nucleotides with a significantly higher phastCons score (Student’s t-test; p < 2.2e-16). Error bars represent the SEM.