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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Methods Mol Biol. 2015;1221:149–170. doi: 10.1007/978-1-4939-1571-2_12

Figure 1.

Figure 1

Strategy for cloning the full-length cDNA of the HRV-16 genome. The genome structure of HRV-16 is shown on the top. The NdeI and BlpI restriction enzyme sites were used for cloning. Viral RNA was extracted from infected cell lysate and converted into cDNA. Three pairs of PCR primers (F1/R1, F2/R2 and F3/R3) were used to generate three overlapping PCR fragments (A, B and C). Each PCR fragment was cloned and the plasmid isolates containing the correct sequences were identified by restriction analysis and sequencing. Then the fragments A, B and C were assembled into a full-length clone with NdeI and BlpI restriction sites.