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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Methods Mol Biol. 2015;1221:149–170. doi: 10.1007/978-1-4939-1571-2_12

Figure 2.

Figure 2

T7 in vitro transcripts of HRV16 full-length cDNA. T7 in vitro transcripts were made as described in the Method section 3.2.1. Virion RNA was extracted from purified virions as described in the Method section 3.1.3. The concentration of virion RNA was measured with OD260nm (1 OD = 40 μg/ml). The RNAs were electrophoresed in a 0.8% agarose/1x TBE gel and the gel was stained with EtBr. Lane 1: 1 μg virion RNA, lane 2: 4 μl T7 transcripts, and Lane 3: 2 μl T7 transcripts.