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. 2015 May 6;14:101. doi: 10.1186/s12943-015-0373-6

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© Liu et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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MEFs null for Klf4 exhibit lower levels of autophagy compared to the wild-type during starvation. (A) Morphology of Klf4 ^+/+ and Klf4 ^−/− MEFs with time course EBSS treatment. Klf4 ^+/+ and Klf4 ^−/− MEFs were treated with EBSS for the durations indicated and photographed with an Olympus IX51 inverted microscope. Cell lysates from Klf4 ^+/+ (B) and Klf4 ^−/− (C) MEFs were analyzed using Western blot. Antibodies against LC3B were used to indicate autophagy levels and β-Actin was used as a loading control. (D) Densitometry analysis of LC3B bands shown in (B) and (C). The intensities of LC3BII bands were normalized to that of β-actin using Gel Imager program. Error bars represent standard error. The experiments were repeated 3 times. * indicates p < 0.05, ** indicates p < 0.01.