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. 2015 May 6;14:101. doi: 10.1186/s12943-015-0373-6

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© Liu et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Impaired autophagy in Klf4 ^−/− MEFs results in increased DNA damage. Klf4 ^+/+ and Klf4 ^−/− MEFs were treated with full-media or EBSS for 8 hrs. (A) Immunostaining against γ-H2AX (green), a DNA damage marker, was performed to assess the levels of DNA damage in Klf4 ^+/+ and Klf4 ^−/− MEFs. DAPI (blue) was used to visualize the nuclei. (B) The percentage of Klf4 ^+/+ and Klf4 ^−/− MEFs cultured in full-media or EBSS with less than 5, 5—15, or more than 15 γ-H2AX foci was quantified. At least 300 MEFs were counted for each condition. * indicates p < 0.05, ** indicates p < 0.01. (C) Western blot analysis was performed to assay the levels of γ-H2AX. The experiments were repeated 3 times.