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. 2015 May 6;10(5):e0126653. doi: 10.1371/journal.pone.0126653

Fig 5. Let-7b/cytotoxin combination reduces migration and invasion of KRAS mutant tumor cells.

Fig 5

A, the effect of let-7b mimic, paclitaxel or gemcitabine on cell migration was evaluated in A549 and Panc-1 cells at 0, 12 and 24 hours following the scratch wound (images, left panel). The area of wound healing was quantified by ImageJ (right panel). B, the matrigel invasion assay was conducted to evaluate the effect of let-7b mimic, paclitaxel or gemcitabine on the invasiveness of A549 and Panc-1 cells. Images were acquired using an inverted microscope at × 40 magnification. C, Protein levels of HMGA2, Snail 1, E-cadherin and vimentin in A549 and Panc-1 cells were assessed by Western blotting. Each experiment was carried out at least 3 times. Data represent the mean ± SEM. *, p < 0.05; **, p < 0.01.