Table 1. Effect of let-7b restoration on the cytotoxicity of paclitaxel and gemcitabine in KRAS mutant (MT) and wide-type (WT) cell lines.
IC50 of Paclitaxel | IC50 of Gemcitabine | |||||
---|---|---|---|---|---|---|
+ scramble | + let-7b | CI | + scramble | + let-7b | CI | |
A549 (KRAS MT) | 21 ± 1 nM | 4 ±1 nM | 0.46 | 92 ± 2 nM | 25 ± 1 nM | 0.57 |
H1975 (KRAS WT) | 11 ± 1 nM | 9 ±1 nM | 1.04 | 48 ± 1 nM | 37 ± 1 nM | 0.98 |
Panc-1 (KRAS MT) | 25 ± 1 nM | 8 ± 1 nM | 0.36 | 36 ± 1 μM | 17 ± 1 μM | 0.47 |
BxPC-3 (KRAS WT) | 22 ± 1 nM | 17 ±1 nM | 1.01 | 30 ± 1 nM | 22 ± 1 nM | 0.96 |
CI: combination index; CI < 0.9 indicates synergism; CI = 0.9–1.1 indicates additivity, and CI > 1.1 indicates antagonism.