Skip to main content
. Author manuscript; available in PMC: 2015 May 6.
Published in final edited form as: Nature. 2015 Jan 21;518(7537):55–60. doi: 10.1038/nature14121

Extended Data Figure 4.

Extended Data Figure 4

Western blot analysis of tyrS.d7 variants.

Variants of tyrS.d7 with leucine or tryptophan at the bipA position were expressed as GST fusions under identical conditions and analyzed by Western blot (Methods). Soluble protein content was quantified by densitometry and normalized to GAPDH. Mutating bipA to leucine or tryptophan reduced soluble TyrS levels by 2.5- or 2.1-fold, respectively (p < 0.05 by two-tailed unpaired student’s t-test with unequal variances). Three technical replicates were performed; a representative image is shown.