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. 2015 May 2;13:15. doi: 10.1186/s12953-015-0071-8

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© Wang et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Immunoblot and quantitative RT-PCR analysis of the 20 representative DEPs. The expression profile for Rubisco was detected using 1-D Western blot analysis (A). The identified three different members of Rubisco large subunit genes were up-regulated at transcriptional level in the Bt-transgenic lines (B). The changed patterns of genes for the five DEPs related to photosynthesis were determined (C). The gene expression patterns of the other 12 representative DEPs in both the Bt-transgenic (right) and non-transgenic (left) plants were highlighted (D). The primers used for qRT-PCR were provided in Additional file 6.