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. 2015 Apr 30;12:39. doi: 10.1186/s12977-015-0167-3

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© Serrao et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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BRD4 protein and interaction with Rev-A IN. (A) Schematic of human BRD4 isoform C (NCBI reference sequence NP_055114.1) highlighting various protein domains and the 426–720 fragment used in this study. (B) Ni-NTA pull-down of purified BRD4_462–720 by His₆-tagged Rev-A IN. Migration positions of standards (in kDa) are labeled to the left. Lanes 1–4: 20% reaction input of the indicated proteins. Lanes 5 and 6: the indicated BRD4_462–720 protein was incubated with Ni-NTA beads in the absence of Rev-A IN. Lanes 7 and 8: the indicated BRD4_462–720 protein was incubated with Rev-A IN-bound beads. The gel is representative of results obtained from three independent experiments.