Figure 7.

Concerted integration activity of Rev-A IN. (A) Ethidium bromide stained image of Rev-A IN integration reactions in the presence of increasing concentrations of indicated BRD4_462–720 protein. Migration positions of standards (in kb) are shown to the left, and the positions of single vDNA end, or half-site, and concerted vDNA integration products, as well as supercoiled (s.c.) and open circular (o.c) forms of the plasmid tDNA, are to the right. Lanes 1 and 2: Rev-A IN and tDNA incubated without vDNA or with vDNA, respectively. Lanes 3–6: increasing concentrations (0.05, 0.15, 0.35, 0.5 μM) of BRD4_462–720 incubated with Rev-A IN plus vDNA and tDNA. Lanes 7–10: same as lanes 3–6 but with BRD4_{462–720/L630E}. (B) Strand transfer activities for three independent experiments ± standard error of the mean, measured by quantification of DNA band intensity. The results were normalized to the level of Rev-A IN concerted integration activity in the absence of BRD4_462–720 proteins, which was set to 100%. P values of <0.05 and <0.01 are indicated by * and **, respectively, as determined by one-tailed t-test.