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. 2015 May 6;11(5):e1004853. doi: 10.1371/journal.ppat.1004853

Fig 2. B. abortus infection induces PMN cell death in a dose dependent manner.

Fig 2

(A) Heparinized blood was incubated with B. abortus-GFP (MOI 10) for two hours and PMNs population analyzed for cell death using AquaDead and Annexin V markers. GFP fluorescence intensity was used to differentiate among three categories: (a) low, (b) intermediate and (c) high infection. (B) Percentages of PMNs positive for any marker in relation to the number of internalized bacteria are shown. Experiments were repeated at least three times.