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. 2015 May 6;11(5):e1004853. doi: 10.1371/journal.ppat.1004853

Fig 8. Brucella lipid A induces PMNs cell death in a dose dependent manner.

Fig 8

(A) Heparinized blood was incubated for two hours with LPSs of Y. enterocolitica O:9 (3 pmol/mL), of E. coli (7.5 pmol/mL), of B. abortus 2308 (3 pmol/mL), of B. abortus ∆WadC (3 pmol/mL) and of O. anthropi (2 pmol/mL), all corresponding to 100μg/mL of LPS. The LPSs differed in at least one of the moieties (O-chain, core and lipid A) with B. abortus 2308 LPS: (a) LPSs possessing lipid As that differ from B. abortus 2308 LPS, (b) LPSs possessing lipid As structures similar to B. abortus 2308 LPS. (B) Heparinized blood treated with different concentrations of B. abortus 2308 lipid A for two hours. In all assays, PMN population was gated and analyzed by Annexin V marker and the geometric means of histograms displayed as relative units. Experiments were repeated at least three times.