Fig 6. Puberty in IR/LepRKiss mice.
(A) Balanopreputial separation age in male control (n = 12), IRKiss (n = 10) and IR/LepRKiss (n = 3) mice. Estimated age of sexual maturation calculated by subtraction of 20 days from the time required for delivery of a litter sired by male control (n = 8), IRKiss (n = 10) and IR/LepRKiss (n = 4) mice. (B) Vaginal opening and first estrus was evaluated in female control (n = 9–12), IRKiss (n = 10) and IR/LepRKiss (n = 7–8) mice. (C) LH serum levels on postnatal day 31 in male control (n = 7) and IR/LepRKiss (n = 7) mice and female control (n = 10) and IR/LepRKiss (n = 6) mice. (D) FSH serum levels on postnatal day 31 in male control (n = 7) and IR/LepRKiss (n = 7) mice and in female control (n = 10) and IR/LepRKiss (n = 6) mice.