Fig 8. Analysis of metabolites after over-expression of TbMCP14 in procyclic forms.

A) A volcano plot of metabolites identified in cells over-expressing TbMCP14 reveals that a metabolite with a mass consistent with it being pyrroline-5-carboxylate was the only metabolite showing a greater than 3-fold change in abundance with statistical significance (p<0.05). B) Pyrroline-5-carboxylate intensities in lysates from parasites cultured in the presence (+ tet) or absence (- tet) of tetracycline to over-express TbMCP14. The asterisks represent statistical difference relative to the respective uninduced controls (**p<0.01, unpaired student’s t test).