Fig 9. Effect of knock-down of TbMCP14 on growth of T. brucei procyclic forms cultured in glucose-depleted medium.

A) Parasites were grown in SDM80 containing normal (5.5 mM; panel A) or low (0.15 mM; panel B) levels of glucose in the absence (black squares) or presence (red circles) of tetracycline to induce RNAi-mediated down-regulation of TbMCP14. C,D) TbMCP14 conditional knock-out parasites were grown in glucose-depleted (0.15 mM, panel C) or glucose-containing SDM80 (5.5 mM, panel D) in the presence (black squares, +MCP14) or absence (red circles,-MCP14) of tetracycline to maintain or deplete, respectively, TbMCP14. All data points represent mean values ± standard deviations from three independent experiments; for some data points, the error bars are smaller than the symbols.