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. Author manuscript; available in PMC: 2015 May 6.
Published in final edited form as: Clin Cancer Res. 2013 Nov 15;20(1):187–198. doi: 10.1158/1078-0432.CCR-13-1279

Figure 2.

Figure 2

Neutrophils promote the proliferation of GICs. A, Morphology of GFP-positive GICs with and without co-culture with MPRO cells (at 1:1 ratio) for 3 days. (100X) Bar graph demonstrating the fold change of GFP-expressing cell numbers analyzed by Flow Cytometry. B, Fold changes of cell numbers after 3 days incubation of GIC11 or GIC23 with serum-free condition media (CM) collected from MPRO cells. Cell cycle analysis using propidium iodide (PI) staining showed CM decreased the percentages of cells in sub-G1 stages (apoptotic cells, C) and increased that in G2/M+S stages (growing cells, D). E, Expression of cyclin D1, cyclin D2, C-myc and Tubulin were detected by western blots in GICs incubated with or without CM. *: P<0.05; **: P<0.01, Student’s t test.