Table 4. LNs-binding preference for hGal1, 3 and 7.

	LN1/LN2 ratio 1
Protein	Current study	Previous study 4
hGal1	0.44 2 (0.53 3 )	0.79
hGal3	0.35 2 (0.23 3 )	0.65 5
hGal7	1.52 2 (2.97 3 )	2.7
hGal3-E165A	1.22 2

¹To quantitatively evaluate LN1- or LN2-binding preference of hGal1, 3 and 7, LN1/LN2 ratios (K _d ^LN1 /K _d ^LN2) were calculated based on the K _d values presented in Table 1.

²The values were obtained based on the K _d ^BI values determined by Biolayer Interferometry.

³The values were obtained based on the K _d ^FP values determined by FP-based competition assays.

⁴It was reported by Hirabayashi, J. et al. [12].

⁵The CRD domain was used instead of full-length hGal3.