Table 3. Charges distribution, inter-domain salt bridges and H-bonds in the 3D models of APEHs from T. bernacchii and C. hamatus.

	T. bernacchii				C. hamatus
	APEH-1		APEH-2		APEH-1		APEH-2
	N-ter	C-ter	N-ter	C-ter	N-ter	C-ter	N-ter	C-ter
Sequence region	1–450	461–728	1–420	431–690	1–450	461–729	1–420	431–690
Total negative charges (D+E)	64	29	42	24	56	29	46	24
Total positive charges(H+K+R)	58	26	51	31	57	27	53	31
Net charge	-6	-3	9	7	1	-2	7	7
Total Protein net charge	-9		16		-1		14
Total Protein Salt bridges	20		20		16		11
Inter-domain salt bridges	2		2		4		5
Total Protein H-bonds	445		465		482		456
Inter-domain H-bonds	33		57		48		46