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. 1987 Aug;80(2):566–572. doi: 10.1172/JCI113105

Influences of variation in total energy intake and dietary composition on regulation of fat cell lipolysis in ideal-weight subjects.

H Kather, E Wieland, A Scheurer, G Vogel, U Wildenberg, C Joost
PMCID: PMC442270  PMID: 3301902

Abstract

Weight-maintaining fat-rich, "prudent," carbohydrate-rich, as well as energy-restricted diets (300 kcal/d) were fed in succession for 7 d to 12 healthy males of ideal body weight under metabolic ward conditions. At the end of each period isolated fat cells were prepared from subcutaneous abdominal adipose tissue and incubated in vitro in the absence or presence of adenosine deaminase, either alone or in combination with various lipolytic or antilipolytic hormones and agents. Variations in total energy intake and dietary composition had characteristic and specific effects on fat cell lipolysis in vitro. High carbohydrate and prudent diets resulted in low rates of nonstimulated glycerol release and impaired insulin action in the presence of adenosine deaminase (320 mU/ml). High-fat and energy restricted diets were characterized by high rates of nonstimulated glycerol release. Sensitivity of antilipolysis to insulin and prostaglandin E2 was 10 to 200 times lower respectively on energy-restricted than on fat-rich diets. The effects of alpha 2- and beta-adrenergic catecholamines and of N6-phenylisopropyladenosine were not affected by the preceding diets.

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Selected References

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  1. Arner P., Bolinder J., Engfeldt P., Hellmér J., Ostman J. Influence of obesity on the antilipolytic effect of insulin in isolated human fat cells obtained before and after glucose ingestion. J Clin Invest. 1984 Mar;73(3):673–680. doi: 10.1172/JCI111259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Arner P., Bolinder J., Engfeldt P., Ostman J. The antilipolytic effect of insulin in human adipose tissue in obesity, diabetes mellitus, hyperinsulinemia, and starvation. Metabolism. 1981 Aug;30(8):753–760. doi: 10.1016/0026-0495(81)90020-2. [DOI] [PubMed] [Google Scholar]
  3. Arner P., Bolinder J., Ostman J. Marked increase in insulin sensitivity of human fat cells 1 hour after glucose ingestion. J Clin Invest. 1983 Mar;71(3):709–714. doi: 10.1172/JCI110817. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Berlan M., Lafontan M. Evidence that epinephrine acts preferentially as an antilipolytic agent in abdominal human subcutaneous fat cells: assessment by analysis of beta and alpha 2 adrenoceptor properties. Eur J Clin Invest. 1985 Dec;15(6):341–348. doi: 10.1111/j.1365-2362.1985.tb00282.x. [DOI] [PubMed] [Google Scholar]
  5. Chang J., Lewis G. P., Piper P. J. Inhibition by glucocorticoids of prostaglandin release from adipose tissue in vitro. Br J Pharmacol. 1977 Mar;59(3):425–432. doi: 10.1111/j.1476-5381.1977.tb08396.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chohan P., Carpenter C., Saggerson E. D. Changes in the anti-lipolytic action and binding to plasma membranes of N6-L-phenylisopropyladenosine in adipocytes from starved and hypothyroid rats. Biochem J. 1984 Oct 1;223(1):53–59. doi: 10.1042/bj2230053. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Denton R. M., Brownsey R. W., Belsham G. J. A partial view of the mechanism of insulin action. Diabetologia. 1981 Oct;21(4):347–362. doi: 10.1007/BF00252681. [DOI] [PubMed] [Google Scholar]
  8. Galster A. D., Clutter W. E., Cryer P. E., Collins J. A., Bier D. M. Epinephrine plasma thresholds for lipolytic effects in man: measurements of fatty acid transport with [l-13C]palmitic acid. J Clin Invest. 1981 Jun;67(6):1729–1738. doi: 10.1172/JCI110211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. German D. C., Kredich N. M. A radioenzymatic assay for plasma adenosine. Anal Biochem. 1984 Nov 1;142(2):536–541. doi: 10.1016/0003-2697(84)90503-7. [DOI] [PubMed] [Google Scholar]
  10. Gilman A. G. G proteins and dual control of adenylate cyclase. Cell. 1984 Mar;36(3):577–579. doi: 10.1016/0092-8674(84)90336-2. [DOI] [PubMed] [Google Scholar]
  11. Giudicelli Y., Lacasa D., Agli B. Alterations induced by a prolonged fasting: opposite effects on the beta-adrenergic receptor-coupled adenylate-cyclase system and on lipolysis in fat cells from rat. Eur J Biochem. 1982 Jan;121(2):301–308. doi: 10.1111/j.1432-1033.1982.tb05786.x. [DOI] [PubMed] [Google Scholar]
  12. Gorman M. W., Bassingthwaighte J. B., Olsson R. A., Sparks H. V. Endothelial cell uptake of adenosine in canine skeletal muscle. Am J Physiol. 1986 Mar;250(3 Pt 2):H482–H489. doi: 10.1152/ajpheart.1986.250.3.H482. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Grundy S. M. Comparison of monounsaturated fatty acids and carbohydrates for lowering plasma cholesterol. N Engl J Med. 1986 Mar 20;314(12):745–748. doi: 10.1056/NEJM198603203141204. [DOI] [PubMed] [Google Scholar]
  14. Hales C. N., Luzio J. P., Siddle K. Hormonal control of adipose-tissue lipolysis. Biochem Soc Symp. 1978;(43):97–135. [PubMed] [Google Scholar]
  15. Honnor R. C., Dhillon G. S., Londos C. cAMP-dependent protein kinase and lipolysis in rat adipocytes. I. Cell preparation, manipulation, and predictability in behavior. J Biol Chem. 1985 Dec 5;260(28):15122–15129. [PubMed] [Google Scholar]
  16. Honnor R. C., Dhillon G. S., Londos C. cAMP-dependent protein kinase and lipolysis in rat adipocytes. II. Definition of steady-state relationship with lipolytic and antilipolytic modulators. J Biol Chem. 1985 Dec 5;260(28):15130–15138. [PubMed] [Google Scholar]
  17. Kahn C. R. Insulin resistance, insulin insensitivity, and insulin unresponsiveness: a necessary distinction. Metabolism. 1978 Dec;27(12 Suppl 2):1893–1902. doi: 10.1016/s0026-0495(78)80007-9. [DOI] [PubMed] [Google Scholar]
  18. Kashiwagi A., Bogardus C., Lillioja S., Huecksteadt T. P., Brady D., Verso M. A., Foley J. E. In vitro insensitivity of glucose transport and antilipolysis to insulin due to receptor and postreceptor abnormalities in obese Pima Indians with normal glucose tolerance. Metabolism. 1984 Aug;33(8):772–777. doi: 10.1016/0026-0495(84)90221-x. [DOI] [PubMed] [Google Scholar]
  19. Kather H., Bieger W., Michel G., Aktories K., Jakobs K. H. Human fat cell lipolysis is primarily regulated by inhibitory modulators acting through distinct mechanisms. J Clin Invest. 1985 Oct;76(4):1559–1565. doi: 10.1172/JCI112137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Kather H., Wieland E., Fischer B., Schlierf G. Antilipolytic effects of N6-phenylisopropyladenosine and prostaglandin E2 in fat-cells of obese volunteers before and during energy restriction. Biochem J. 1985 Nov 1;231(3):531–535. doi: 10.1042/bj2310531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Kather H., Wieland E., Fischer B., Wirth A., Schlierf G. Adrenergic regulation of lipolysis in abdominal adipocytes of obese subjects during caloric restriction: reversal of catecholamine action caused by relief of endogenous inhibition. Eur J Clin Invest. 1985 Feb;15(1):30–37. doi: 10.1111/j.1365-2362.1985.tb00140.x. [DOI] [PubMed] [Google Scholar]
  22. Kohlmeier M., Stricker G., Schlierf G. Influences of "normal" and "prudent" diets on biliary and serum lipids in healthy women. Am J Clin Nutr. 1985 Dec;42(6):1201–1205. doi: 10.1093/ajcn/42.6.1201. [DOI] [PubMed] [Google Scholar]
  23. Londos C., Honnor R. C., Dhillon G. S. cAMP-dependent protein kinase and lipolysis in rat adipocytes. III. Multiple modes of insulin regulation of lipolysis and regulation of insulin responses by adenylate cyclase regulators. J Biol Chem. 1985 Dec 5;260(28):15139–15145. [PubMed] [Google Scholar]
  24. Ontyd J., Schrader J. Measurement of adenosine, inosine, and hypoxanthine in human plasma. J Chromatogr. 1984 May 11;307(2):404–409. doi: 10.1016/s0378-4347(00)84113-4. [DOI] [PubMed] [Google Scholar]
  25. Opmeer F. A., Adolfs M. J., Bonta I. L. Regulation of prostaglandin E2 receptors in vivo by dietary fatty acids in peritoneal macrophages from rats. J Lipid Res. 1984 Mar;25(3):262–268. [PubMed] [Google Scholar]
  26. Pedersen O., Hjøllund E., Sørensen N. S. Insulin receptor binding and insulin action in human fat cells: effects of obesity and fasting. Metabolism. 1982 Sep;31(9):884–895. doi: 10.1016/0026-0495(82)90177-9. [DOI] [PubMed] [Google Scholar]
  27. RODBELL M. METABOLISM OF ISOLATED FAT CELLS. I. EFFECTS OF HORMONES ON GLUCOSE METABOLISM AND LIPOLYSIS. J Biol Chem. 1964 Feb;239:375–380. [PubMed] [Google Scholar]
  28. Sahyoun N., LeVine H., 3rd, Stenbuck P., Cuatrecasas P. Cytosolic activator of adenylate cyclase: Reconstitution, characterization, and mechanism of action. Proc Natl Acad Sci U S A. 1983 Jun;80(12):3646–3650. doi: 10.1073/pnas.80.12.3646. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Shane E., Yeh M., Feigin A. S., Owens J. M., Bilezikian J. P. Cytosol activator protein from rat reticulocytes requires the stimulatory guanine nucleotide-binding protein for its actions on adenylate cyclase. Endocrinology. 1985 Jul;117(1):255–263. doi: 10.1210/endo-117-1-255. [DOI] [PubMed] [Google Scholar]
  30. Sjöström L., Schutz Y., Gudinchet F., Hegnell L., Pittet P. G., Jéquier E. Epinephrine sensitivity with respect to metabolic rate and other variables in women. Am J Physiol. 1983 Nov;245(5 Pt 1):E431–E442. doi: 10.1152/ajpendo.1983.245.5.E431. [DOI] [PubMed] [Google Scholar]
  31. Smith U., Cahlin E., Scherstén T. Sucrose feeding in man. Effects on lipolysis and antilipolytic action of insulin in the adipose tissue. Acta Med Scand. 1973;194(3):147–150. [PubMed] [Google Scholar]

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