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. 2015 May 6;11(5):e1004256. doi: 10.1371/journal.pcbi.1004256

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© 2015 Toufighi et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — (A) Experimental transcriptome analysis of the different process [3]. Differentiation was induced in human primary stem cells with 1.2mM CaCl2. Cells were collected every 5 hours in triplicates during 45 hours, total RNA was extracted from each sample, synthesized into cDNA, amplified in cRNA, Cy3 labeled, and hybridized to microarrays. Data were collected, normalized and gens were classified based on expression profiles. (B) Clusters of gene expression dynamics along differentiation. The 1317 expressed super-dynamic genes were clustered using K-means clustering and the proportion of the different functional categories is shown in the pie diagrams below. See also the legend.