Fig 4. Evaluation of integrins αL, αM, αX, αD and β2 mRNA expression in gut K^dGag_197–205–specific CD8⁺ T cells following i.n./i.n., i.n./i.m. and i.m./i.m. vaccination.

The log mRNA expression normalised to housekeeping gene L32 of integrin αL (a), αM (b), αX (c), αD (d), β2 (e) αD (black circle) and β2 (inverted red triangle) (f) and αX (green circle), αM (black circle) which can pair up with β2 (inverted red triangle) (g) is shown. The p values were calculated using one-way ANOVA. In all the plots circles or triangles or square indicate a pool of hundred gut K^dGag_197–205-specific CD8⁺ T cells and line indicate mean expression. The experiment was repeated three times. Data represent the three experiments.