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. 2015 May 6;10(5):e0125774. doi: 10.1371/journal.pone.0125774

Fig 7. Characterization of the effects of GMI in MDR lung cancer sublines.

Fig 7

(A) JC-1 assay to measure the mitochondrial membrane potential at 24 h of GMI treatment in both sublines. (B) Analysis of apoptosis on Annexin V assay (C) and autophagy by AVO formation in cells treated with GMI (1.2 μM). (D) A549/V16 cells were pretreated with pan-caspase inhibitor Z-VAD-FMK (50 μM) for 1 h followed by exposure to various concentrations of GMI for 48 h and analysis on MTT assay. (F) Protein levels in cells were analyzed on Western blot (E) shLuc and shATG5-74 of A549/V16 cells were treated with various concentrations of GMI for 48 h then analyzed on MTT assay. (G) Protein levels in cells were analyzed on Western blot. (H) Detection of the LC3A-II and c-PARP occurrence at 16 h and 24 h of GMI treatment in A549/D16 subline and (I) A549/V16 subline.