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. 2015 May 6;10(5):e0123600. doi: 10.1371/journal.pone.0123600

Fig 3. RNAi-mediated silencing of TGF-2 inhibits clonogenic growth in UMSCC25 cells.

Fig 3

2 independent shRNA sequences targeting TGF-β2 or a non-silencing control were transduced into UMSCC25 cells using a lentiviral construct to create 3 cell lines: shTGFB2.4, shTGFB2.5, and NSC. A. qRT-PCR was performed to demonstrate that TGF-β2 mRNA was decreased with the shRNA compared to the NSC. B. An ELISA performed on media from the knockdown cells demonstrates decreased TGF-β2 protein secretion into the media. C. Representative images of the clonogenic assay where cells were treated with DMSO, 0.3μM AZD8010, 0.1μM gefinitib, or both for 2 weeks. D. Graphical presentation of the quantified results from the clonogenic assay. ***denotes a p-value <0.0001.