Table 1. Search results from in silico mining of a de novo transcriptome from Calanus finmarchicus using glutathione S-transferase (GST) queries obtained from the copepod Tigriopus japonicus, the cladoceran Daphnia pulex and the insect Drosophila melanogaster.
Class | Subclass | Transcript accession number | Transcript length† |
---|---|---|---|
Cytosolic | Delta/Theta/Epsilon* | GAXK01204954 | 888 |
GAXK01204965 | 921 | ||
GAXK01204950 | 852 | ||
GAXK01204940 | 965 | ||
GAXK01204947 | 1182 | ||
GAXK01204957 | 902 | ||
GAXK01204968 | 764 | ||
GAXK01204953 | 991 | ||
GAXK01073468 | 401 | ||
GAXK01096295 | 914 | ||
GAXK01035521 | 823 | ||
Mu | GAXK01204944 | 957 | |
GAXK01204956 | 741 | ||
GAXK01204948 | 742 | ||
GAXK01204952 | 1686 | ||
GAXK01204958 | 785 | ||
Omega | GAXK01204960 | 984 | |
GAXK01016325 | 1410 | ||
GAXK01164502 | 894 | ||
Sigma | GAXK01204949 | 1346 | |
GAXK01204945 | 839 | ||
GAXK01204943 | 815 | ||
GAXK01204951 | 733 | ||
GAXK01204959 | 955 | ||
GAXK01204946 | 1022 | ||
GAXK01204964 | 771 | ||
GAXK01204961 | 884 | ||
GAXK01204942 | 756 | ||
GAXK01204966 | 881 | ||
Zeta | GAXK01204939 | 1033 | |
GAXK01204941 | 2790 | ||
GAXK01084871 | 359 | ||
Mitochondrial | Kappa | GAXK01046934 | 1108 |
Microsomal | mGST-1 | GAXK01178264 | 771 |
GAXK01081966 | 347 | ||
mGST-3 | GAXK01204963 | 54 | |
GAXK01204967 | 465 | ||
GAXK01204962 | 7866 | ||
GAXK01204955 | 680 |
Transcript lengths (base pairs) are given for each transcript identified.
†Length in nucleotides.
Accession Nos for query proteins: GST Delta T. japonicus (ACE81244, ACE81245). GST Theta T. japonicus (ACE81253). GST Epsilon D. melanogaster (AAF57701). GST Mu T. japonicus (ACE81251, ACE81252, ACE81254). GST Omega T. japonicus (ACE81246). GST Sigma T. japonicus (AAY89316). GST Zeta T. japonicus (ACE81250). Mitochondrial GST Kappa D. pulex (EFX86155). Microsomal GST-1 T. japonicus (ACE81248). Microsomal GST-3 T. japonicus (ACE81249) as listed.
*Queries for Delta, Theta and Epsilon subclasses deduced the identical list of 11 transcripts.
C. finmarchicus transcripts whose accession numbers are shown in bold font have sequence support from expressed sequence tag data (see text).