Table 2. Annotation of putative glutathione S-transferase-encoding transcripts from Table 1, using reciprocal BLAST results and protein domain analysis.
| Class—subclass and assigned protein name | Transcript accession No. | Deduced protein length† | Structural domains | Species | Protein accession No. | E-value | % amino acid identity/similarity |
|---|---|---|---|---|---|---|---|
| Cytosolic—Delta | |||||||
| Calfi-Delta-I | GAXK01204953 | 217* | GSTN, GSTC | Lepeophtheirus salmonis | ACO12967 | 8.9e-44 | 40/78 |
| Calfi-Delta-II | GAXK01204968 | 217** | GSTN, GSTC | Lepeophtheirus salmonis | ACO12967 | 8.9e-36 | 43/77 |
| Calfi-Delta-III | GAXK01204940 | 218* | GSTN, GSTC | Lepeophtheirus salmonis | ACO12967 | 7.0e-33 | 39/80 |
| Calfi-Delta-IV | GAXK01204965 | 220** | GSTN, GSTC | Daphnia pulex | EFX81633 | 2.8e-29 | 69/70 |
| Calfi-Delta-V | GAXK01204954 | 221* | GSTN, GSTC | Tigriopus japonicus | ACE81245 | 2.8e-29 | 57/86 |
| Calfi-Delta-VI | GAXK01204957 | 237** | GSTN, GSTC | Lepeophtheirus salmonis | ACO12967 | 1.1e-28 | 38/80 |
| Calfi-Delta-VII | GAXK01204947 | 262* | GSTN, GSTC | Lepeophtheirus salmonis | ADD38060 | 3.2e-25 | 59/89 |
| Calfi-Delta-VIII | GAXK01073468 | 113*** | GSTN | Tigriopus japonicus | ACE81245 | 7.5e-27 | 33/44 |
| Calfi-Delta-IX | GAXK01204950 | 178*** | GSTC | Caligus clemensi | ACO15541 | 3.2e-17 | 44/68 |
| Calfi-Delta-X | GAXK01035521 | 201* | GSTN, GSTC | Caligus rogercresseyi | ACO15749 | 2.2e-10 | 21/54 |
| Calfi-Delta-XI | GAXK01204939 | 330** | GSTN, GSTC | Lepeophtheirus salmonis | ADD38823 | 1.3e-07 | 27/54 |
| Cytosolic—Theta | |||||||
| Calfi-Theta-I | GAXK01096295 | 262** | GSTN, GSTC | Locusta migratoria | AEB91980 | 1.3e-79 | 45/72 |
| Cytosolic—Mu | |||||||
| Calfi-Mu-I | GAXK01204944 | 222* | GSTN, GSTC | Caligus clemensi | ACO15225 | 2.2e-105 | 65/89 |
| Calfi-Mu-II | GAXK01204956 | 222** | GSTN, GSTC | Macrobrachium nipponense | AGJ70295 | 3.1e-95 | 65/85 |
| Calfi-Mu-III | GAXK01204948 | 222** | GSTN, GSTC | Tigriopus japonicus | ACE81254 | 1.0e-90 | 48/69 |
| Calfi-Mu-IV | GAXK01204952 | 222* | GSTN, GSTC | Tigriopus japonicus | ACE81254 | 9.5e-90 | 58/82 |
| Calfi-Mu-V | GAXK01204958 | 222* | GSTN, GSTC | Tigriopus japonicus | ACE81254 | 4.1e-59 | 45/76 |
| Cytosolic—Omega | |||||||
| Calfi-Omega-I | GAXK01204960 | 268* | GSTN, GSTC | Riptortus pedestris | BAN21163 | 5.3e-46 | 36/64 |
| Calfi-Omega-II | GAXK01164502 | 235*** | GSTN | Acromyrmex echinatior | EGI63780 | 3.2e-32 | 35/64 |
| Calfi-Omega-III | GAXK01016325 | 272*** | GSTN | Coptotermes formosanus | AFZ78680 | 2.6e-51 | 36/68 |
| Cytosolic—Sigma | |||||||
| Calfi-Sigma-I | GAXK01204964 | 200** | GSTN, GSTC | Daphnia pulex | EFX82687 | 3.1e-44 | 36/70 |
| Calfi-Sigma-II | GAXK01204942 | 204* | GSTN, GSTC | Daphnia pulex | EFX82672 | 2.0e-42 | 40/67 |
| Calfi-Sigma-III | GAXK01204943 | 216** | GSTN, GSTC | Tigriopus japonicus | AAY89316 | 7.2e-45 | 40/74 |
| Calfi-Sigma-VI | GAXK01204959 | 217* | GSTN, GSTC | Apis florae | XP_003694330 | 8.2e-38 | 35/71 |
| Calfi-Sigma-V | GAXK01204951 | 218* | GSTN, GSTC | Tigriopus japonicus | AAY89316 | 5.0e-33 | 38/70 |
| Calfi-Sigma-VI | GAXK01204961 | 218**** | GSTN, GSTC | Daphnia pulex | EFX82672 | 5.1e-39 | 38/69 |
| Calfi-Sigma-VII | GAXK01204946 | 225* | GSTN, GSTC | Daphnia pulex | EFX63772 | 2.1e-39 | 38/76 |
| Calfi-Sigma-VIII | GAXK01204966 | 239* | GSTN, GSTC | Daphnia pulex | EFX82672 | 1.8e-62 | 45/75 |
| Calfi-Sigma-XI | GAXK01204949 | 196* | GSTN, GSTC | Folsomia candida | AGZ95070 | 1.2e-35 | 35/51 |
| Calfi-Sigma-II | GAXK01204945 | 223* | GSTN, GSTC | Megachile rotundata | XP_00370954 | 1.1e-37 | 36/56 |
| Cytosolic—Zeta | |||||||
| Calfi-Zeta-I | GAXK01204941 | 225** | GSTN, GSTC | Drosophila melanogaster | NP_731358 | 5.0e-84 | 54/82 |
| Calfi-Zeta-II | GAXK01084871 | 113*** | GSTC | Bactrocera dorsalis | AFI99067 | 6.1e-27 | 22/32 |
| Mitochondrial—Kappa | |||||||
| Calfi-Kappa-I | GAXK01046934 | 256* | THX | Paracyclopina nana | ADV59554 | 1.2e-65 | 36/61 |
| Microsomal—mGST-1 | |||||||
| Calfi-mGST-1-I | GAXK01081966 | 93*** | MAPEG | Drosophila persimilis | XP_002023020 | 4.0e-42 | 36/61 |
| Calfi-mGST-1-II | GAXK01081966 | 93*** | MAPEG | Ceratitis capitata | XP_00453691 | 3.2e-26 | 31/52 |
| Microsomal—mGST-3 | |||||||
| Calfi-mGST-3-I | GAXK01204963 | 156** | MAPEG | Acartia pacifica | AGN29624 | 3.2e-60 | 65/84 |
| Calfi-mGST-3-II | GAXK01204967 | 156** | MAPEG | Daphnia pulex | EFX85348 | 7.1e-37 | 46/77 |
| Calfi-mGST-3-III | GAXK01204955 | 264** | MAPEG | Daphnia pulex | EFX85347 | 2.2e-34 | 42/73 |
| Calfi-mGST-3-IV | GAXK01204962 | 145**** | MAPEG | Acartia pacifica | AGN29624 | 5.1e-49 | 61/75 |
BLAST searches were limited to NCBI non-redundant protein database for arthropods (taxid: 6656).
†Length in amino acids.
* Predicted full-length protein flanked by “stop” codons at both N- and C-terminals.
** Putative full-length protein flanked by a “methionine” at the N-terminal, and a “stop” codon at the C-terminal. Identification of full-length is based on presence of expected structural domains and similarity to full-length proteins.
*** Partial protein with either the N-terminal “methionine” or C-terminal “stop” codon missing.
**** Protein originally identified as full-length, but prediction corrected to partial after alignment with their most similar transcript in the Norwegian Sea transcriptome (see text).
Abbreviations: GSTN, GST amino (N)-terminal domain; GSTC, GST carboxyl (C)-terminal domain; THX, thioredoxin-like domain; MAPEG, membrane-associated proteins in eicosanoid and glutathione metabolism domain.