Figure 2. Comparisons of RP-HPLC fractionation of P. pacificus pyridylamino-labelled N-glycans.

Chromatograms obtained with pyridylaminated N-glycans released either with PNGase F alone (A) or with PNGase A after PNGase F (B) are shown for the Ascentis® Express RP-amide, Agilent Hypersil ODS C18 and Phenomenex Kinetex™ XB-C18 columns; the chromatograms are normalised with respect to the elution of the Man_8,9GlcNAc₂ and Man₃GlcNAc₂Fuc₁ glycans and the calibration in terms of glucose units is shown. The chromatograms are also annotated with the structures of selected N-glycans with either similar or contrasting relative elution times with boxes in the same form (colour, shading or dashes) to highlight the elution position of the same glycan in different chromatograms.