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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Electrophoresis. 2015 Jun;36(11-12):1314–1329. doi: 10.1002/elps.201400528

Figure 4. MALDI-TOF MS/MS of pyridylamino-labelled mono-, di- and tri-fucosylated N-glycans.

Figure 4

Examples of MS/MS spectra for glycans monofucosylated on the reducing-terminal GlcNAc (A,B), monofucosylated on the distal GlcNAc (C), difucosylated on the reducing-terminal GlcNAc (D,F), fucosylated on both the reducing-terminal and distal GlcNAc (E), galactosylated on core fucose (G) or trifucosylated on the core (H,I) are shown for glycans identified in various RP-HPLC fractions. Key fragments are annotated with abbreviations of the form HxNyFz where H is hexose, N N-acetylhexosamine, and F fucose or with diagrammatic depictions, in which the α1,3- and α1,6-fucose residues are respectively shown in the ‘down’ or ‘up’ positions.