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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Electrophoresis. 2015 Jun;36(11-12):1314–1329. doi: 10.1002/elps.201400528

Figure 7. MALDI-TOF MS analysis of isomers of pyridylamino-labelled N-glycans with non-reducing N-acetylglucosamine residues.

Figure 7

Selected C18 RP-HPLC fractions (A, C) were treated with either the specific FDL β-hexosaminidase (B) or with α1,2/3-mannosidase (D) in order to reveal whether GlcNAc residues were present on the α1,3-arm (i.e., the GlcNAc transferred by GlcNAc-TI). All glycans in these fractions were subject to MS/MS and examples for two isomers of Man3GlcNAc3 and the single isomer of Man3GlcNAc5 are shown (E-G). The residues cleaved enzymatically or the bonds putatively cleaved during MS/MS fragmentation are indicated with dashed lines; losses of N-acetylglucosamine or α1,3-mannose are indicated by the Δ values.