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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Electrophoresis. 2015 Jun;36(11-12):1314–1329. doi: 10.1002/elps.201400528

Figure 8. MALDI-TOF MS/MS of pyridylamino-labelled N-glycans modified with phosphorylcholine.

Figure 8

The fragmentation patterns for selected glycans predicted to be modified with phosphorylcholine and present in various fractions of PNGase F or PNGase A released pools chromatographed with the Kinetex XB-C18 column are shown: (A,B) one isomer each of glycans with m/z 1357 and m/z 1503, (C,D) two differently-eluting isomers with m/z 1560, (E) one isomer with m/z 1763, (F) the single isomer with m/z 2093 and (G) the single isomer with m/z 1649. The typical fragments of m/z 369, 531, 572 and 734 are diagnostic for Hex0-1HexNAc1-2PC1.