Table I. List of predicted N-glycans for Pristionchus pacificus.
Glycans released by either PNGase F (F) or PNGase A after F (A), whose structures were proven on the basis of retention time (in glucose units for the three columns used), MS/MS fragmentation pattern and sensitivity/resistance to chemical and enzymatic treatments, are shown according to the nomenclature of the Consortium for Functional Glycomics; calculated m/z for pyridylamino-labelled glycans (as [M+H]+) are presented as well as, for some structures, the ‘Schachter-style’ abbreviation.
| Structure | m/z | Retention time | ||
|---|---|---|---|---|
| RPC18 | RP-amide | Kinetex | ||
|
665.28 | A/F 6.4 | A/F 6.2 | F 7.8 |
|
811.35 | A 4.3 | A 4.3 | A 4.9 |
|
811.35 | A/F 7.7 | F 7.5 | A/F 11.5 |
|
811.35 | A/F 10.3 | A/F 8.2 | A/F 13.5 |
|
827.34 (0M) | A/F 5.9 | A/F 6.1 | A/F 7.5 |
|
827.34 (M0) | A/F 7.7 | A/F 7.2 | A/F 9.5 |
|
957.40 | A 5.9 | n.d. | A 6.8 |
|
957.40 | A 7.0 | A 6.3 | A 8.3 |
|
957.40 | n.d. | A 8.7 | F > 25.0 |
|
971.42 | A 19.0 | A 9.5 | A > 25 |
|
973.40 (0MF3) | A 4.2 | A 4.5 | A 4.7 |
|
973.40 (M0F3) | A 5.0 | A 5.0 | A 5.5 |
|
973.40 | A/F 7.7 | F 7.2 | A/F 10.2 |
|
973.40 (0MF6) | A/F 9.8 | A/F 8.2 | A/F 13 |
|
973.40 (M0F6) | A/F 13.0 | A/F 9.0 | A/F 16 |
|
989.39 (MM) | A/F 7.2 | A/F 7.2 | A/F 9.5 |
|
1030.42 (0Gn) | A/F 6.5 | A/F 6.1 | A/F 7.5 |
|
1119.46 | A 5.2 | A 5.4 | A 6.8 |
|
1119.46 | A 7.0 | A 6.3 | A 8.3 |
|
1119.46 | F 13.0 | n.d. | F > 25.0 |
|
1135.45 (MMF3) | A 5.0 | A 5.1 | A 5.5 |
|
1135.45 (MMF6) | A/F 11.5 | A/F 9.5 | A/F 16.0 |
|
1176.48 | n.d. | F 8.2 | F 13.0 |
|
1151.45 | A/F 7.2 | A/F 7.2 | A/F 9.5 |
|
1192.47 (MGn) | A/F 7.0 | A/F 6.8 | A/F 8.4 |
|
1192.47 (GnM) | A/F 10.3 | A/F 9.0 | F/A 16.0 |
|
1195.47 | A/F 7.7 | A/F 5.5 | A/F 6.9 |
|
1265.51 | A 8.0 | A 6.8 | A 9.2 |
|
1265.51 | A 9.8 | A 8.0 | A 11.2 |
|
1281.51 (MMF3F6) | A 7.2 | A 6.8 | A 8.5 |
|
1297.50 | A 5.0 | A 5.0 | A 5.8 |
|
1297.50 | n.d. | F 11.0 | A/F > 20.0 |
|
1313.50 (Man5) | A/F 7.0 | A/F 7.2 | A/F 8.7 |
|
1338.53 (MGnF6) | A/F 10.3 | A/F 8.6 | A/F 13.0 |
|
1338.53 (GnMF6) | F 17.0 | F 12.0 | A/F > 25.0 |
|
1341.53 | F 13.0 | F/A 7.7 | A/F 11.5 |
|
1357.53 | A/F 8.0 | A/F 6.2 | A/F 7.5 |
|
1357.53 | A/F 11.5 | A/F 8.17 | A/F 14.5 |
|
1395.55 (GnGn) | A/F 9.0 | A/F 8.10 | A/F 11.5 |
|
1427.56 | A 11.5 | A 8.2 | A 12.7 |
|
1459.56 | A 4.7 | A 5.0 | A 5.4 |
|
1475.55 | A/F 5.5 | A/F 6.1 | A/F 6.5 |
|
1475.55 | A/F 5.9 | A/F 6.3 | A/F 6.9 |
|
1484.59 | A 9.0 | A 7.8 | A 11.2 |
|
1503.59 | A/F 13.0 | A/F 8.0 | A/F 11.5 |
|
1503.59 | A/F 19.0 | A/F 11.0 | F > 25.0 |
|
1541.61 (GnGnF6) | F 14.0 | F 10.5 | F 21.0 |
|
1560.61 | F 8.0 | F 6.8 | F 8.8 |
|
1560.61 | F 10.3 | F 7.5 | F 9.5 |
|
1598.63 | A/F 6.5 | F 6.3 | F 7.5 |
|
1637.60 | A/F 5.0 | A/F 5.5 | A/F 5.3 |
|
1637.60 | A/F 5.7 | A/F 6.1 | A/F 6.2 |
|
1649.64 | A/F 9.8 | A 7.2 | A 10.0 |
|
1706.67 | F 17.0 | F 9.5 | F 16.0 |
|
1725.66 | A/F 15.0 | A/F 6.8 | A/F 9.5 |
|
1744.69 | n.d. | F 8.2 | F 11.5 |
|
1763.69 | F 7.2 | F 5.5 | F 6.3 |
|
1763.69 | n.d. | F 5.9 | F 6.9 |
|
1799.66 (Man8B) | A/F 4.6 | A/F 5.2 | A/F 5.0 |
|
1799.66 (Man8A) | A/F 5.3 | F 5.8 | A/F 6.0 |
|
1871.72 | A/F > 20 | F 8.6 | F 14.5 |
|
1909.75 | n.d. | F 6.8 | F 8.8 |
|
1928.74 | F 17.0 | F 4.0 | F 5.0 |
|
1928.74 | n.d. | n.d. | F 5.7 |
|
1961.71 (Man9) | A/F 4.9 | A/F 5.5 | A/F 5.3 |
|
2074.80 | F > 20.0 | n.d. | F 6.9 |
|
2074.80 | n.d. | F 6.3 | F 8.0 |
|
2093.80 | A/F > 20.0 | A/F 4.8 | F 4.8 |
|
2239.86 | F > 20.0 | F 4.5 | F 6.7 |