Table 2.
A list of highly confident identifications of glycopeptides from the rhEPO with the predicted structures, glycosylation sites and the relative abundance from log2 (peak areas).
| ID | Glycan structure | Relative abundance(%) | |||
|---|---|---|---|---|---|
| N24 | N38 | N83 | O126 | ||
| 1 | (Hex)0(HexNAc)1(NeuAc)1(NeuGc)0(Deoxyhex)1+(Man)3(GlcNAc)2 | 0.4 | |||
| 2 | (Hex)1(HexNAc)1(NeuAc)1(NeuGc)0(Deoxyhex)0 | 53.5 | |||
| 3 | (Hex)1(HexNAc)1(NeuAc)1(NeuGc)0(Deoxyhex)1+(Man)3(GlcNAc)2 | 2.3 | 0.5 | 0.0 | |
| 4 | (Hex)1(HexNAc)1(NeuAc)2(NeuGc)0(Deoxyhex)0 | 46.5 | |||
| 5 | (Hex)1(HexNAc)1(NeuAc)4(NeuGc)0(Deoxyhex)1+(Man)3(GlcNAc)2 | 0.2 | |||
| 6 | (Hex)1(HexNAc)2(NeuAc)1(NeuGc)0(Deoxyhex)1+(Man)3(GlcNAc)2 | 1.2 | |||
| 7 | (Hex)1(HexNAc)2(NeuAc)3(NeuGc)0(Deoxyhex)0+(Man)3(GlcNAc)2 | 0.3 | |||
| 8 | (Hex)2(HexNAc)2(NeuAc)0(NeuGc)1(Deoxyhex)1+(Man)3(GlcNAc)2 | 0.1 | |||
| 9 | (Hex)2(HexNAc)2(NeuAc)1(NeuGc)0(Deoxyhex)1+(Man)3(GlcNAc)2 | 13.1 | 3.1 | ||
| 10 | (Hex)2(HexNAc)2(NeuAc)2(NeuGc)0(Deoxyhex)1+(Man)3(GlcNAc)2 | 28.7 | 6.2 | 1.1 | |
| 11 | (Hex)2(HexNAc)2(NeuAc)3(NeuGc)0(Deoxyhex)0+(Man)3(GlcNAc)2 | 0.2 | |||
| 12 | (Hex)2(HexNAc)2(NeuAc)3(NeuGc)0(Deoxyhex)1+(Man)3(GlcNAc)2 | 0.5 | |||
| 13 | (Hex)2(HexNAc)3(NeuAc)1(NeuGc)1(Deoxyhex)1+(Man)3(GlcNAc)2 | 0.5 | |||
| 14 | (Hex)2(HexNAc)3(NeuAc)2(NeuGc)0(Deoxyhex)1+(Man)3(GlcNAc)2 | 2.3 | 0.3 | ||
| 15 | (Hex)2(HexNAc)3(NeuAc)2(NeuGc)2(Deoxyhex)0+(Man)3(GlcNAc)2 | 0.3 | |||
| 16 | (Hex)3(HexNAc)3(NeuAc)1(NeuGc)0(Deoxyhex)1+(Man)3(GlcNAc)2 | 10.0 | 4.3 | ||
| 17 | (Hex)3(HexNAc)3(NeuAc)1(NeuGc)1(Deoxyhex)1+(Man)3(GlcNAc)2 | 0.2 | |||
| 18 | (Hex)3(HexNAc)3(NeuAc)2(NeuGc)0(Deoxyhex)1+(Man)3(GlcNAc)2 | 11.8 | 11.1 | 4.2 | |
| 19 | (Hex)3(HexNAc)3(NeuAc)3(NeuGc)0(Deoxyhex)1+(Man)3(GlcNAc)2 | 7.2 | 2.7 | 3.0 | |
| 20 | (Hex)3(HexNAc)4(NeuAc)1(NeuGc)0(Deoxyhex)0+(Man)3(GlcNAc)2 | 0.6 | |||
| 21 | (Hex)3(HexNAc)4(NeuAc)2(NeuGc)0(Deoxyhex)1+(Man)3(GlcNAc)2 | 0.4 | |||
| 22 | (Hex)4(HexNAc)4(NeuAc)1(NeuGc)0(Deoxyhex)1+(Man)3(GlcNAc)2 | 2.4 | |||
| 23 | (Hex)4(HexNAc)4(NeuAc)2(NeuGc)0(Deoxyhex)1+(Man)3(GlcNAc)2 | 4.3 | 10.4 | 7.3 | |
| 24 | (Hex)4(HexNAc)4(NeuAc)3(NeuGc)0(Deoxyhex)0+(Man)3(GlcNAc)2 | 0.1 | |||
| 25 | (Hex)4(HexNAc)4(NeuAc)3(NeuGc)0(Deoxyhex)1+(Man)3(GlcNAc)2 | 1.2 | 10.5 | 9.7 | |
| 26 | (Hex)4(HexNAc)4(NeuAc)4(NeuGc)0(Deoxyhex)1+(Man)3(GlcNAc)2 | 1.0 | 2.8 | 0.7 | |
| 27 | (Hex)4(HexNAc)5(NeuAc)3(NeuGc)0(Deoxyhex)0+(Man)3(GlcNAc)2 | 1.1 | |||
| 28 | (Hex)5(HexNAc)5(NeuAc)1(NeuGc)0(Deoxyhex)0+(Man)3(GlcNAc)2 | 0.3 | 2.0 | 36.9 | |
| 29 | (Hex)5(HexNAc)5(NeuAc)1(NeuGc)0(Deoxyhex)1+(Man)3(GlcNAc)2 | 1.9 | 0.6 | ||
| 30 | (Hex)5(HexNAc)5(NeuAc)2(NeuGc)0(Deoxyhex)1+(Man)3(GlcNAc)2 | 2.5 | 9.1 | ||
| 31 | (Hex)5(HexNAc)5(NeuAc)3(NeuGc)0(Deoxyhex)1+(Man)3(GlcNAc)2 | 3.5 | |||
| 32 | (Hex)5(HexNAc)5(NeuAc)4(NeuGc)0(Deoxyhex)1+(Man)3(GlcNAc)2 | 0.7 | 1.9 | ||
| 33 | (Hex)6(HexNAc)6(NeuAc)1(NeuGc)0(Deoxyhex)0+(Man)3(GlcNAc)2 | 1.3 | 15.6 | 6.1 | |
| 34 | (Hex)6(HexNAc)6(NeuAc)1(NeuGc)0Deoxyhex1+(Man)3(GlcNAc)2 | 2.8 | |||
| 35 | (Hex)6(HexNAc)6(NeuAc)2(NeuGc)0(Deoxyhex)0+(Man)3(GlcNAc)2 | 0.6 | 6.3 | 0.6 | |
| 36 | (Hex)6(HexNAc)6(NeuAc)3(NeuGc)0(Deoxyhex)1+(Man)3(GlcNAc)2 | 0.2 | |||
| 37 | (Hex)6(HexNAc)6(NeuAc)4(NeuGc)0(Deoxyhex)1+(Man)3(GlcNAc)2 | 2.6 | 0.8 | ||
| 38 | (Hex)7(HexNAc)7(NeuAc)0(NeuGc)2(Deoxyhex)0+(Man)3(GlcNAc)2 | 0.1 | |||
| 39 | (Hex)7(HexNAc)7(NeuAc)1(NeuGc)0(Deoxyhex)0+(Man)3(GlcNAc)2 | 2.3 | 9.1 | 17.8 | |
| 40 | (Hex)7(HexNAc)7(NeuAc)1(NeuGc)0(Deoxyhex)1+(Man)3(GlcNAc)2 | 0.3 | |||
| 41 | (Hex)7(HexNAc)7(NeuAc)2(NeuGc)0(Deoxyhex)0+(Man)3(GlcNAc)2 | 0.2 | |||
| 42 | (Hex)7(HexNAc)7(NeuAc)4(NeuGc)0(Deoxyhex)1+(Man)3(GlcNAc)2 | 0.1 | |||
| 43 | (Hex)7(HexNAc)8(NeuAc)1(NeuGc)0Deoxyhex1+(Man)3(GlcNAc)2 | 0.6 | |||
| 44 | (Hex)8(HexNAc)8(NeuAc)1(NeuGc)0(Deoxyhex)0+(Man)3(GlcNAc)2 | 0.9 | |||
| 45 | (Hex)8(HexNAc)8(NeuAc)2(NeuGc)0(Deoxyhex)0+(Man)3(GlcNAc)2 | 0.1 | |||
| 46 | (Hex)9(HexNAc)9(NeuAc)1(NeuGc)0(Deoxyhex)0+(Man)3(GlcNAc)2 | 3.8 | |||
| total | 100.0 | 100.0 | 100.0 | 100.0 | |