Figure 1.

(Scheme 1) A standard protocol for glycoprotein analysis. Purification and derivatization (permethylation) methods are commonly necessary to increase analytical sensitivity, resulting in a time consuming and complex procedure.⁵ (Scheme 2) Previously reported protocol for the simultaneous omics approach using IM-MS.³ This protocol allows both peptides and N-linked glycans to be simultaneously analyzed with minimal sample preparation. Sensitivity gains are afforded by the use of IM-MS. (Scheme 3) The protocol describes the separation and analysis of carbohydrates without modification by IM-MS. In this scheme, glycoproteins are subjected to denaturing by heat followed by PNGase F enzyme incubation prior to analysis. This procedure simplifies the interpretation of carbohydrates without derivatization or further purification.