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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Ann Rheum Dis. 2014 Nov 6;75(1):295–302. doi: 10.1136/annrheumdis-2014-205790

Figure 2.

Figure 2

Receptor protein tyrosine phosphatase κ (RPTPκ) is required for rheumatoid arthritis (RA) fibroblast-like synoviocyte (FLS) invasiveness. (A and B) Following treatment with control (Ctl) or PTPRK (A) or PTPRK_2 (B) antisense oligonucleotide (ASO) for 7 days, RA FLS invaded through Matrigel-coated transwell chambers in response to 50 ng/mL platelet-derived growth factor BB (PDGF-BB) for 24 h. Graphs show median±IQR % maximum number of cells per field. Data from four (A) or three (B) independent experiments in different FLS lines are shown. (C) ASO-treated RA FLS migrated through uncoated transwell chambers in response to 5% fetal bovine serum (FBS) for 24 h. Graph shows median±IQR % maximum number of cells per field. Data from five independent experiments in different FLS lines are shown. (A–C) *p<0.05, Mann–Whitney test. (D and E) ASO-treated RA FLS migrated out of a Matrigel spot for 2 days in response to 10 ng/mL PDGF or media alone. (D) Median±IQR cells per field. Data from three independent experiments in different FLS lines are shown. *p<0.05, Wilcoxon-matched pairs signed-rank test. (E) Representative image of cells from (D). (F) ASO-treated RA FLS were plated on fibronectin (FN)-coated coverslips in the presence of 5% FBS. Graph shows median±IQR cell area after 15, 30 and 60 min. Data from three independent experiments in different FLS lines are shown. *p<0.05, Wilcoxon-matched pairs signed-rank test.