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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Lab Invest. 2014 Dec 1;95(2):132–141. doi: 10.1038/labinvest.2014.144

Figure 5. Post-operative pericardial fluid primes neutrophils for increased oxidative burst.

Figure 5

Priming of the oxidative burst in normal, healthy donor neutrophils collected from pericardial fluid at 4 hours post-surgery. Pericardial fluid was centrifuged at 1,500xg and then diluted to 2.5%, 5%, and 10% in XF DMEM. It was then added to freshly isolated and plated neutrophils 1.5 hours prior to the assay. A) Baseline OCR was measured in neutrophils prior to inhibition of mitochondrial respiration with antimycin A (10 μM) and induction of the oxidative burst with PMA (100 ng/mL). B) The area under the curve (AUC) during the 48 minute oxidative burst response was calculated and plotted by PCF dose. *= p<0.05, **= p<0.005. 3-5 replicated wells per group.