Figure 1.

Schematic of workflow developed to analyze the effect of CSD in WT and R192Q mouse brains. Ninety-six coronal brain sections were obtained from a total of 32 mouse brains (three consecutive sections per animal). Proteins, peptides, and metabolites were independently analyzed by MSI using optimized sample treatment for each molecular class as described in the Materials and Methods section. Each section was stained with Nissl reagent after matrix removal and the MSI datasets, and histologic images were aligned to the Allen Brain Atlas of mouse brain [19]. Automatic anatomical annotation of regions of interest allowed the extraction of MSI data from specific brain regions of interest and statistical analysis