Figure 5.

Evaluation of tumor cell viability in response to combinatorial therapy. Western blot for PARP cleavage and CHOP expression in THJ29T and THJ16T ATC cells treated with A939572 (A) or MF-438 (B) in combination with carfilzomib. β-Actin is shown to demonstrate equal protein loading. C, Cell death analysis using flow cytometry sorting of propidium iodide-stained cells treated with the IC₅₀ dose of indicated drugs after a 48-hour treatment. Car, carfilzomib. Graphical results are shown in panel D. *, 5% or greater increase in cell death as compared with DMSO control; **, 5% or greater increase in cell death as compared with monotherapy.