Figure 4.

Annexin V and PI staining for the apoptosis assay in Bxpc-3 and PANC-1 cells after 48 h of treatment with amiloride, erlotinib, or their combination. (A) Apoptosis was assayed by Annexin V and PI staining and fluorescence-activated cell sorting analysis. (B) The percentage of early and late apoptotic cells was indicated in the histogram. ^bP<0.05, ^cP<0.01 compared with control. ^eP<0.05 compared with groups treated with erlotinib alone. (C) Western blot analysis of cleaved PARP (p85) expression confirms the apoptotic effects of the drug combination.