Figure 5.

Effects of amiloride on EGF (20 ng/mL) simulated phosphorylation of EGFR, Akt, and Erk in Bxpc-3, PANC-1, Aspc-1, and CFPAC-1 cells. Cells were pre-treated with different concentrations of amiloride for 2 h and then stimulated with 20 ng/mL EGF for 30 min, harvested and analyzed by Western blot analysis. (A) The experiment was independently repeated three times and representative results are shown in graphs. (B) Band intensities were quantified and the ratio of gray values for p-EGFR, p-Akt, and p-Erk was analyzed using β-actin as a control. Mean±SD. n=3. ^bP<0.05, ^cP<0.01 compared with control.