Fig. 2. EGCG treatment increases AMPK phosphorylation. (A and B) H9C2 cardiomyocytes were treated with various concentrations of EGCG for 24 h. The levels of phosphor-/total AMPK were detected. (C and D) Cells were treated with 25µM EGCG for indicated time points and the protein expression of phosphor-/total AMPK were assessed by western blotting. (E and F) Cells were preincubated with 25µM EGCG for 1 h followed by stimulation with 100µM PE for 24 h, and then the Levels of phosphor-/total AMPK were measured by western blotting. *p<0.05 vs. the group without treatment, #p<0.05 vs. the group treated with PE, n=3.
